Population genetic structure of endosymbiotic bacteria of deep-sea hydrothermal vent bivalve mussels

Deep-sea hydrothermal vents along the global mid-ocean ridge systems have provided unique chemosynthetic habitats to a variety of invertebrate animals that depend on chemosynthetic bacteria for their nourishment. This symbiotic association between host animals and their bacterial symbionts keeps intriguing us on their nature and evolution. While there has been a remarkable progress in our understanding of geographic distributional structure of host invertebrates and its implication, the same quest toward their endosymbionts is very limited, mostly due to a lack of relevant markers and/or inconvenient PCR-cloning method for bacterial population study. Here we present a population genetic approach to understand the geography of genetic variation of sulfur-oxidizing endosymbionts of Bathymodiolus mussels in the East Pacific Rise (EPR) and the Pacific-Antarctic (PAR) Ridge. This investigation includes experimental development of multiple polymorphic markers of bacterial protein-coding genes and their application to all populations simultaneously by parallel 454 pyrosequencing method based on barcodes. Multiple genetic screening of endosymbionts identified a salient genetic disconnection at the Easter Microplate across where host mussels species and other vent invertebrates previously have shown marked divergence, and monotonous connection at either side of the genetic break. Such a corresponding genetic pattern at either vent invertebrates or the present endosymbionts around the boundary of the EPR and PAR suggest that the two recognized biogeographic provinces have a profound influence on not only vent animals but also vent microbes.

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First Name: 
Yong-Jin
Last Name: 
Won
Telephone: 
82 2 3277 4471
Affiliation: 
Division of EcoScience, Ewha Womans University, Seoul, 120-750 Korea
First Name: 
Eunji
Last Name: 
Park
Affiliation: 
Division of EcoScience, Ewha Womans University, Seoul, 120-750 Korea
First Name: 
Ye-Seul
Last Name: 
Kwan
Affiliation: 
Division of EcoScience, Ewha Womans University, Seoul, 120-750 Korea
First Name: 
Phuong Thao
Last Name: 
Ho
Affiliation: 
Division of EcoScience, Ewha Womans University, Seoul, 120-750 Korea
First Name: 
Sook-Jin
Last Name: 
Jang
Affiliation: 
Division of EcoScience, Ewha Womans University, Seoul, 120-750 Korea
First Name: 
Soon Gyu
Last Name: 
Hong
Affiliation: 
Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, 406-840 Korea
Choose keywords that are most applicable to your abstract. (Three maximum.): 
Biogeography
Abstract ID: 
CBE5-145